Biological Background of LARP6

LARP6 (La-related protein 6) is a member of the La-related protein superfamily, a conserved family of RNA-binding proteins defined by the presence of a La module comprising a La motif and an adjacent RNA recognition motif (RRM). The human LARP6 gene encodes a protein that is predominantly expressed in tissues undergoing active extracellular matrix remodeling, including fibroblasts, hepatic stellate cells, and smooth muscle cells.

The defining functional role of LARP6 lies in its ability to bind specific stem-loop structures located in the 5′ untranslated regions (5′ UTRs) of collagen type I (COL1A1 and COL1A2) and collagen type III mRNAs. This binding event is central to the coordinated post-transcriptional control of collagen biosynthesis, influencing mRNA stability, translational efficiency, and subcellular localization of collagen transcripts on the endoplasmic reticulum membrane.

Beyond collagen regulation, LARP6 has been implicated in broader RNA regulatory networks, interacting with components of the cytoskeleton and RNA transport machinery. Dysregulation of LARP6 expression or activity has been associated with pathological fibrosis in organs such as the liver and lung, making it a subject of significant biomedical interest.

Biochemical Properties of Recombinant Human LARP6 Protein

Recombinant Human LARP6 Protein (SKU: rp375170) is produced using recombinant expression systems to ensure consistent quality and biological relevance. The recombinant protein retains the structural features necessary for RNA-binding activity, including the intact La module responsible for recognizing cognate RNA stem-loop structures.

Key biochemical characteristics of recombinant LARP6 include its RNA-binding specificity, which has been characterized against structured 5′ UTR stem-loop elements of collagen mRNAs. The protein's La module enables high-affinity interactions with these RNA targets, making the recombinant protein a suitable tool for in vitro biochemical assays.

For detailed specifications including purity grade, formulation, molecular weight, storage conditions, and recommended reconstitution protocols, researchers should consult the official product datasheet available from Aladdin Scientific (product reference: absea#PP-8898, SKU: rp375170). Specific numerical values for purity, concentration, and endotoxin levels are provided in the current product datasheet and may vary by lot.

Research Applications

Recombinant Human LARP6 Protein is a versatile reagent suitable for a range of in vitro and cell-based research applications:

• RNA-Protein Interaction Studies: LARP6 is widely used in electrophoretic mobility shift assays (EMSA), RNA pull-down experiments, and surface plasmon resonance (SPR) assays to characterize its binding affinity and specificity for collagen mRNA stem-loop structures and other RNA targets.
• Collagen Biosynthesis Research: As a master regulator of collagen mRNA post-transcriptional control, recombinant LARP6 is employed to dissect the molecular mechanisms governing collagen production, relevant to wound healing, connective tissue biology, and matrix biology research.
• Fibrosis and Disease Modeling: Given the association of LARP6 with fibrotic pathologies, the protein is used in biochemical studies aimed at understanding fibrosis mechanisms in liver, lung, and kidney diseases, providing a basis for target validation in drug discovery programs.
• Structural Biology: Recombinant LARP6 can be used as a component in crystallography, cryo-electron microscopy, or NMR studies aimed at elucidating the structural basis of RNA recognition by the La module.
• Antibody Validation and Assay Development: High-purity recombinant protein serves as a positive control or standard in ELISA, Western blot, and immunoprecipitation assay development and validation workflows.

Storage, Handling, and Quality Considerations

Proper storage and handling of Recombinant Human LARP6 Protein are essential to preserve its biological activity and integrity. Researchers should adhere to the storage conditions specified in the product datasheet provided by Aladdin Scientific. General best practices for recombinant proteins include:

• Storing the protein at the recommended temperature (typically −20°C or −80°C for long-term storage) to prevent degradation.
• Avoiding repeated freeze-thaw cycles, which can reduce protein activity; aliquoting into single-use volumes upon first thaw is recommended.
• Reconstituting lyophilized preparations according to the datasheet instructions, using the recommended buffer to maintain solubility and activity.
• Keeping the protein on ice during experimental manipulation to minimize activity loss.

Aladdin Scientific's recombinant proteins are subject to quality control testing. Specific lot-release criteria, including purity assessment by SDS-PAGE or HPLC, endotoxin testing, and functional activity verification, are documented in the certificate of analysis (CoA) accompanying each lot. Researchers are encouraged to request the CoA for lot-specific data.

Ordering Information

Recombinant Human LARP6 Protein is available from Aladdin Scientific under the following details:

For pricing, available pack sizes, current stock status, and volume discount inquiries, please contact Aladdin Scientific's sales team or visit the product page on the Aladdin Scientific website. Custom formulation or bulk quantity requests can be discussed with our technical sales specialists.