What Is LATS1 and Why Is It Important in Research?

LATS1 (Large Tumor Suppressor Kinase 1) is a serine/threonine kinase and a core effector of the Hippo signaling pathway, a highly conserved pathway that regulates organ size, cell proliferation, and apoptosis. LATS1 phosphorylates and inactivates the transcriptional co-activators YAP (Yes-Associated Protein) and TAZ, promoting their cytoplasmic retention and proteasomal degradation. Dysregulation of LATS1 activity has been implicated in a broad range of human cancers, making it a critical target in oncology research. Recombinant Human LATS1 Protein (rp371987) enables researchers to study these mechanisms in vitro with a well-defined, human-derived reagent.

What Are the Recommended Research Applications for LATS1 Protein?

Recombinant Human LATS1 Protein is suitable for a variety of in vitro biochemical and cell biology research applications. Common uses include:

• In vitro kinase activity assays: Measuring LATS1-mediated phosphorylation of YAP, TAZ, or synthetic peptide substrates.
• Protein interaction studies: Investigating binding partners within the Hippo pathway, such as MOB1 and MST1/2.
• Drug screening and inhibitor profiling: Evaluating the potency and selectivity of small-molecule kinase inhibitors targeting LATS1.
• Antibody validation: Serving as a positive control antigen for western blotting or ELISA experiments.
• Structural and biophysical studies: Supporting biochemical characterization efforts including thermal shift assays and binding kinetics measurements.

Researchers should consult the product datasheet for confirmed and optimized application conditions.

How Should Recombinant Human LATS1 Protein Be Stored and Handled?

Proper storage and handling of Recombinant Human LATS1 Protein are critical to maintaining its activity and integrity. General best practices for recombinant kinase proteins include:

• Storage temperature: Store at -80°C for long-term preservation. Short-term storage at -20°C may be acceptable depending on formulation; refer to the product datasheet for confirmed conditions.
• Avoid freeze-thaw cycles: Repeated freezing and thawing can cause protein aggregation and loss of kinase activity. Aliquot the protein upon first use to minimize freeze-thaw events.
• Reconstitution: If supplied lyophilized, reconstitute with the recommended buffer (typically sterile water or PBS containing a carrier protein such as BSA) as specified in the datasheet.
• Working solutions: Prepare working dilutions fresh and keep on ice during experiments to preserve enzyme activity.
• Contamination prevention: Use sterile, low-binding tubes and pipette tips to prevent adsorption and microbial contamination.

Always refer to the official product datasheet for product-specific storage and handling instructions.

What Quality and Purity Standards Apply to This Recombinant Protein?

Recombinant Human LATS1 Protein (rp371987) is manufactured to research-grade quality standards. Key quality attributes for recombinant proteins of this class typically include:

• Purity: Assessed by SDS-PAGE and/or HPLC; research-grade recombinant proteins are commonly supplied at >85–95% purity. Confirm the specific purity specification in the product datasheet.
• Biological activity: Verified by functional kinase assay (e.g., phosphorylation of a validated substrate such as YAP or a peptide analog).
• Endotoxin levels: Tested and controlled to minimize interference with cell-based assays; specific endotoxin limits are listed on the datasheet.
• Identity confirmation: Confirmed by mass spectrometry or N-terminal sequencing to verify the correct human LATS1 sequence.

For lot-specific quality data including purity, activity, and endotoxin results, please request the Certificate of Analysis (CoA) for your specific lot number from Aladdin Scientific.

How to Order and What to Expect from Aladdin Scientific

Recombinant Human LATS1 Protein is available from Aladdin Scientific under SKU rp371987. To place an order or request additional product information:

• Online ordering: Search for SKU rp371987 on the Aladdin Scientific website to view current pricing, available pack sizes, and stock status.
• Quotations: For bulk quantities or institutional purchasing, contact the Aladdin Scientific sales team for a custom quotation.
• Technical support: For application-specific guidance, buffer recommendations, or troubleshooting assistance, contact Aladdin Scientific technical support.
• Documentation: Product datasheets, Safety Data Sheets (SDS), and Certificates of Analysis (CoA) are available upon request or via the product page.
• Shipping: Recombinant proteins are typically shipped on dry ice to maintain cold-chain integrity; confirm shipping conditions with the sales team at the time of order.

Aladdin Scientific is committed to providing high-quality research reagents with reliable documentation to support reproducible science.