Product Overview and Background

Recombinant Human LCN1P1 Protein (SKU: rp368248) is produced by Aladdin Scientific and is intended for use in research applications. LCN1P1 is annotated as a pseudogene locus related to LCN1 (Lipocalin-1, also known as tear lipocalin), a member of the lipocalin superfamily. Lipocalins are a structurally conserved family of small extracellular proteins known for their ability to bind and transport small hydrophobic molecules such as lipids, retinoids, and steroids.

Pseudogene-derived proteins, when expressed recombinantly, can serve as useful tools for understanding gene family evolution, cross-reactivity in antibody validation, and functional studies aimed at distinguishing paralogous protein activities. Researchers should consult the product datasheet for confirmed sequence information and expression system details prior to experimental use.

Handling, Reconstitution, and Storage Guidelines

Proper handling of recombinant proteins is essential to maintain activity and integrity throughout experimental workflows. The following general guidelines apply to Recombinant Human LCN1P1 Protein; always defer to the specific instructions provided on the Aladdin Scientific product datasheet:

• Reconstitution: If supplied lyophilized, reconstitute in the buffer recommended on the datasheet (typically sterile PBS or a carrier protein-supplemented buffer). Centrifuge the vial briefly before opening to collect the lyophilized material.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize protein degradation caused by repeated freeze-thaw cycles.
• Storage temperature: Store working aliquots at -80 °C for long-term stability. Short-term storage at -20 °C may be acceptable depending on formulation; consult the datasheet.
• Avoid: Repeated freeze-thaw cycles, prolonged exposure to room temperature, and vortexing, all of which may denature the protein.
• Carrier protein: Addition of 0.1% BSA (bovine serum albumin) to the reconstitution buffer can help stabilize dilute protein solutions and reduce adsorption to tube surfaces.

Recommended Applications and Experimental Considerations

Recombinant Human LCN1P1 Protein may be employed in a range of research applications. Researchers are advised to empirically validate the protein for each specific assay format, as performance characteristics can vary by experimental conditions:

• Antibody Validation: Can be used as a positive control or blocking antigen to confirm antibody specificity toward LCN1-related epitopes and to assess cross-reactivity within the lipocalin family.
• ELISA Standards: Suitable as a calibration standard in enzyme-linked immunosorbent assay (ELISA) workflows where LCN1P1-related protein quantification is required.
• Protein–Protein Interaction Studies: May serve as bait or prey protein in pull-down assays or surface plasmon resonance (SPR) experiments to characterize binding partners.
• Structural and Biochemical Studies: Can support studies investigating the structural features conserved across the lipocalin superfamily.

Note: Always confirm application suitability and optimal working concentrations by consulting the product datasheet and conducting pilot experiments.

Quality, Purity, and Regulatory Considerations

Aladdin Scientific recombinant proteins are manufactured under quality-controlled conditions. For Recombinant Human LCN1P1 Protein (rp368248), key quality attributes to review on the product datasheet include:

• Purity: Typical recombinant proteins are purified to >90% as assessed by SDS-PAGE; confirm exact purity specification on the datasheet.
• Endotoxin levels: Endotoxin content should be assessed if the protein is to be used in cell-based or in vivo assays. Check the certificate of analysis (CoA) for lot-specific endotoxin values.
• Expression system: The expression host (e.g., E. coli, baculovirus, mammalian cells) affects post-translational modifications and should be considered when selecting the appropriate protein for your assay.
• Research use only: This product is intended for research purposes only and is not approved for diagnostic or therapeutic use in humans or animals.

For lot-specific quality data, request the Certificate of Analysis (CoA) from Aladdin Scientific at the time of ordering.