What Is LCOR and Why Is It Important in Research?

LCOR (Ligand-Dependent Nuclear Receptor Corepressor) is a nuclear protein that functions as a transcriptional corepressor. It interacts with nuclear receptors in a ligand-dependent manner to suppress the transcription of target genes. LCOR achieves repression by recruiting chromatin-modifying complexes, contributing to epigenetic silencing at specific genomic loci.

Research into LCOR has highlighted its roles in diverse biological processes including hormonal signaling, cell differentiation, and cancer biology. Studies have suggested that LCOR may influence the activity of estrogen receptors and other nuclear hormone receptors, making it a relevant target in oncology and endocrinology research. Recombinant Human LCOR Protein enables researchers to investigate these mechanisms in vitro with a well-defined, consistently produced reagent.

Product Specifications and Quality

Recombinant Human LCOR Protein (SKU: rp369072) is manufactured to research-grade standards. The protein is produced using recombinant expression technology to ensure consistent batch-to-batch quality suitable for biochemical and cell-based assays.

Key quality attributes researchers should verify on the product datasheet include: expression host system (e.g., E. coli, baculovirus, or mammalian cells), purity as determined by SDS-PAGE or HPLC, biological activity (where applicable), and endotoxin levels. Researchers are encouraged to consult the full product datasheet available from Aladdin Scientific for lot-specific values, as these parameters can vary by lot.

Note: Specific numerical values for purity percentage, molecular weight tag, and activity units are not reproduced here to avoid inaccuracy; please refer to the official product datasheet (referenced as absea#PP-11145) or contact Aladdin Scientific technical support for confirmed specifications.

Handling, Storage, and Reconstitution

Proper handling of Recombinant Human LCOR Protein is essential to maintain its integrity and activity. Upon receipt, inspect the product and store it immediately under the conditions specified on the product label and datasheet.

• Short-term storage: If the protein will be used within a few days, it may be stored at 4°C. Prolonged storage at 4°C is not recommended.
• Long-term storage: Store at -80°C. Aliquoting the protein into single-use volumes prior to freezing is strongly recommended to avoid repeated freeze-thaw cycles, which can cause protein degradation and loss of activity.
• Reconstitution: If supplied in lyophilized form, reconstitute according to the datasheet instructions, typically using sterile water or an appropriate buffer. Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex.
• Carrier protein: Adding BSA (bovine serum albumin) to diluted aliquots can help stabilize the protein during storage at low concentrations.

Always centrifuge the vial briefly before use to collect the contents. Follow your institution's biosafety guidelines when handling recombinant proteins.

Research Applications of Recombinant Human LCOR Protein

Recombinant Human LCOR Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: Investigating how LCOR interacts with nuclear receptors (e.g., estrogen receptor, retinoic acid receptor) and other coregulatory proteins using pull-down assays, co-immunoprecipitation, or surface plasmon resonance.
• Biochemical assays: As a substrate or binding partner in ELISA, Western blot validation, or activity assays designed to dissect corepressor mechanisms.
• Structural studies: Providing purified protein for crystallography or cryo-EM studies aimed at resolving the structure of LCOR complexes.
• Drug discovery and screening: Identifying small molecules that modulate LCOR–receptor interactions, particularly relevant in hormone-sensitive cancer research.
• Antibody production and validation: Serving as an immunogen or positive control antigen for antibody generation and specificity testing.

Researchers should confirm suitability for specific assay formats with Aladdin Scientific technical support prior to use.

Ordering and Technical Support

Recombinant Human LCOR Protein is available from Aladdin Scientific under SKU rp369072. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, assay optimization, or protein handling, Aladdin Scientific's technical support team is available to assist. Please have your SKU and lot number ready when contacting support to ensure the fastest and most accurate response.

Bulk quantities and custom formulations may be available upon request. Certificate of Analysis (CoA) and Safety Data Sheet (SDS) documents can be downloaded from the product page or requested from customer service.