Background: MCC Protein Biology

The Mutated in Colorectal Cancer (MCC) gene encodes a cytoplasmic protein that was originally identified through its frequent somatic mutation and loss of expression in colorectal carcinomas. MCC is classified as a tumor suppressor and plays a role in regulating cell cycle progression and apoptosis. Studies have implicated MCC in the negative regulation of the Wnt/β-catenin signaling pathway, a pathway critical to cell fate, proliferation, and differentiation. Loss of MCC function has been observed not only in colorectal cancer but also in other malignancies, suggesting a broader role in oncogenesis. Recombinant Human MCC Protein provides researchers with a well-defined, purified source of this protein for in vitro biochemical and cell biology studies.

Protein Specifications and Quality Considerations

Recombinant Human MCC Protein (SKU: rp373773) is produced using recombinant expression systems to ensure consistency and reproducibility across experiments. When evaluating this protein for use in your assays, the following specification parameters are important to consider:

• Species: Human (Homo sapiens)
• Protein identity: Should be confirmed by SDS-PAGE and/or Western blot prior to experimental use.
• Purity: Recombinant proteins at research grade are typically assessed by SDS-PAGE; consult the product datasheet (absea#PP-7628) for lot-specific purity data.
• Endotoxin level: Critical for cell-based assays; verify endotoxin specifications on the certificate of analysis (CoA).
• Formulation buffer: Review the CoA for the exact buffer composition to ensure compatibility with downstream assays.

Always request and review the lot-specific CoA and datasheet before use to confirm that all parameters meet your experimental requirements.

Handling, Reconstitution, and Storage Protocol

Proper handling of Recombinant Human MCC Protein is essential to maintain biological activity and experimental reproducibility. Follow the guidelines below:

1. Upon receipt: Inspect the vial for integrity. If the protein is supplied lyophilized, briefly centrifuge the vial before opening to consolidate the material at the bottom.
2. Reconstitution: Reconstitute lyophilized protein in the buffer recommended in the product datasheet (typically sterile water or a phosphate-buffered saline-based buffer). Allow the vial to sit at room temperature for a few minutes after addition of buffer and mix gently — do not vortex.
3. Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize protein degradation from repeated freeze-thaw cycles.
4. Short-term storage: If the protein will be used within 1–2 weeks, store aliquots at 4°C.
5. Long-term storage: Store at -80°C. The addition of a carrier protein (e.g., 0.1% BSA) may enhance stability during long-term storage — refer to the datasheet for specific recommendations.
6. Avoid: Repeated freeze-thaw cycles, exposure to temperatures above 25°C, and prolonged exposure to light if the protein is tagged with a fluorescent label.

Note: Always consult the lot-specific product datasheet for reconstitution volumes and storage conditions, as these may vary between lots.

Research Applications

Recombinant Human MCC Protein is suitable for a range of in vitro research applications, including but not limited to:

• Protein–protein interaction studies: MCC has been reported to interact with components of the Wnt signaling pathway, including β-catenin. Pull-down assays and co-immunoprecipitation experiments can be performed using this recombinant protein to map interaction domains.
• Biochemical assays: Use as a positive control or standard in ELISA and Western blot experiments designed to detect or quantify MCC protein levels.
• Structural biology: As a purified recombinant source, this protein may serve as material for biophysical characterization studies.
• Functional rescue experiments: Recombinant MCC protein can be introduced into MCC-deficient cell lines to study the functional consequences of MCC restoration on Wnt signaling or cell proliferation.
• Drug discovery: Used as a target protein for screening small molecules or antibodies that modulate MCC function or its interactions with binding partners.

Researchers are encouraged to validate the protein's activity in their specific assay system using appropriate positive and negative controls.

Troubleshooting Common Issues

If you encounter problems when working with Recombinant Human MCC Protein, consider the following troubleshooting guidance:

For unresolved issues, contact Aladdin Scientific technical support, providing the product SKU (rp373773), lot number, and a detailed description of your experimental conditions.