What Is MCRS1 and Why Is It Important?

MCRS1 (Microspherule Protein 1), also known as MSP58, is a nuclear protein encoded by the MCRS1 gene in humans. It has been implicated in multiple cellular processes including chromatin remodeling, ribosome biogenesis, and cell cycle progression. MCRS1 is known to associate with components of chromatin remodeling complexes and has been reported to interact with the INO80 complex, linking it to DNA damage response and transcriptional regulation.

Research interest in MCRS1 has grown due to its potential roles in oncogenesis; aberrant expression of MCRS1 has been observed in several cancer types, making it a candidate biomarker and therapeutic target. As a recombinant human protein, Aladdin Scientific's rp375212 enables researchers to perform controlled in vitro studies of MCRS1 function, protein–protein interactions, and enzyme activity assays.

Product Specifications and Quality Standards

Aladdin Scientific's Recombinant Human MCRS1 Protein (SKU: rp375212) is manufactured to research-grade standards. Key considerations for this product include:

• Expression System: The specific expression system (e.g., E. coli, baculovirus, mammalian) should be confirmed from the product datasheet, as it affects post-translational modifications and functional activity.
• Purity: Recombinant proteins in this category are typically assessed by SDS-PAGE and/or HPLC; expected purity is generally >85–95% for research-grade reagents, though the exact specification should be verified on the product datasheet.
• Formulation: The buffer composition, presence of carrier proteins (e.g., BSA), and any stabilizers should be reviewed in the datasheet prior to use in sensitive assays.
• Endotoxin Level: For cell-based assays, endotoxin content is a critical specification; consult the product datasheet for lot-specific values.

Researchers are advised to always consult the most current Certificate of Analysis (CoA) provided with their lot for exact specifications.

Handling, Storage, and Reconstitution

Proper handling and storage of recombinant proteins is essential to maintain biological activity and reproducibility. General best-practice guidelines for Recombinant Human MCRS1 Protein include:

• Storage Temperature: Recombinant proteins are typically stored at –20°C or –80°C for long-term stability. Avoid repeated freeze–thaw cycles, which can cause aggregation and loss of activity.
• Aliquoting: Upon first thaw, it is recommended to prepare single-use aliquots to minimize freeze–thaw cycles.
• Reconstitution: If supplied lyophilized, reconstitute according to the datasheet instructions using the recommended diluent (commonly sterile water or a defined buffer). Allow the vial to equilibrate at room temperature briefly before reconstitution.
• Working Concentration: Optimize working concentrations empirically for each assay format. Pilot experiments are advised before committing to large-scale studies.

Always refer to the product-specific datasheet and CoA for lot-specific storage conditions and stability data.

Research Applications of MCRS1 Protein

Recombinant Human MCRS1 Protein supports a range of experimental approaches in molecular and cell biology research:

• Protein–Protein Interaction Studies: MCRS1 can be used as bait or prey in pull-down assays, co-immunoprecipitation validation, and surface plasmon resonance (SPR) experiments to map its interactome.
• Biochemical Activity Assays: Functional studies of MCRS1 involvement in chromatin remodeling complexes may employ recombinant protein in in vitro reconstitution assays.
• Antibody Validation: Recombinant MCRS1 serves as a positive control or immunogen surrogate for validating anti-MCRS1 antibodies in Western blot, ELISA, or dot blot formats.
• Structural Studies: High-purity recombinant protein can support crystallography or cryo-EM sample preparation for structural biology investigations.
• Cancer Biology Research: Given MCRS1's reported association with tumor progression, recombinant protein may be used in assays probing oncogenic mechanisms.

Ordering and Customer Support

Recombinant Human MCRS1 Protein is available from Aladdin Scientific under SKU rp375212. To place an order or request additional technical information:

• Visit the Aladdin Scientific website and search by SKU or product name.
• Contact our technical support team for questions regarding suitability for specific applications, custom formulation inquiries, or bulk pricing.
• Request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for your lot through the website or by contacting customer service.
• For returns, replacements, or quality complaints, please refer to Aladdin Scientific's return and refund policy available on the website.

Our scientific support team is available to assist with experimental design questions related to MCRS1 protein use in your research workflows.