What Is MDH1B and Why Is It Important in Research?

MDH1B (Malate Dehydrogenase 1B) is a cytoplasmic isoform of the malate dehydrogenase enzyme family. Malate dehydrogenases catalyze the reversible conversion of L-malate to oxaloacetate coupled with the reduction of NAD+ to NADH, a reaction central to the citric acid cycle (TCA cycle) and cellular energy metabolism. The cytoplasmic MDH1B isoform plays a role in the malate-aspartate shuttle, which transfers reducing equivalents across the inner mitochondrial membrane, thereby linking cytoplasmic glycolysis with mitochondrial oxidative phosphorylation.

Research interest in MDH1B has grown in the context of metabolic reprogramming in cancer, neurological disorders, and cardiovascular disease. Recombinant production of MDH1B enables researchers to study enzyme kinetics, screen inhibitors or activators, and investigate protein-protein interactions in a controlled biochemical setting without the variability associated with tissue extraction.

Storage, Handling, and Reconstitution of Recombinant Human MDH1B Protein

Proper storage and handling are essential to preserve the activity and integrity of Recombinant Human MDH1B Protein. As a general best practice for recombinant proteins:

• Short-term storage (up to 1 month): Store at 4°C in the supplied buffer.
• Long-term storage: Aliquot to avoid repeated freeze-thaw cycles and store at −20°C or −80°C. Repeated freeze-thaw cycles can lead to loss of enzymatic activity and protein aggregation.
• Reconstitution: If supplied lyophilized, reconstitute in sterile deionized water or the recommended buffer (typically PBS or Tris-based buffer, pH 7.4). Allow the vial to equilibrate to room temperature before opening. Gently mix; do not vortex vigorously.
• Carrier proteins: Addition of a carrier protein such as BSA (0.1–0.5% final concentration) to the working solution may help stabilize dilute preparations.

Note: Please refer to the specific Certificate of Analysis (CoA) provided with lot rp373259 for confirmed storage conditions, buffer composition, and reconstitution volumes, as these may vary by lot.

Typical Research Applications for Recombinant Human MDH1B Protein

Recombinant Human MDH1B Protein (rp373259) is suitable for a range of in vitro research applications, including but not limited to:

• Enzyme activity assays: Measuring MDH1B-catalyzed conversion of malate to oxaloacetate spectrophotometrically by monitoring NADH absorbance at 340 nm.
• Inhibitor and activator screening: High-throughput or focused screening of small molecules that modulate MDH1B activity, relevant to metabolic disease drug discovery.
• Protein-protein interaction studies: Pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance (SPR) experiments.
• Structural biology: Use as a standard or reference in crystallography, cryo-EM, or NMR studies of MDH family members.
• Antibody validation: Positive control antigen for Western blot, ELISA, or immunofluorescence antibody validation.
• Metabolic flux studies: In vitro reconstitution of metabolic pathways involving the malate-aspartate shuttle.

This protein is intended for research use only and is not suitable for diagnostic or therapeutic applications.

Quality, Purity, and What to Expect from Your Order

Aladdin Scientific's Recombinant Human MDH1B Protein (rp373259) is manufactured under controlled conditions designed to deliver consistent purity and activity. While the specific Certificate of Analysis (CoA) values should be consulted for each production lot, researchers should generally expect:

• Purity: Recombinant proteins in this category are typically assessed by SDS-PAGE and may be validated by Western blot using an anti-MDH1B or anti-tag antibody. Target purity levels are generally ≥85% or higher, depending on the purification process.
• Endotoxin levels: Endotoxin testing (LAL assay) is typically performed to ensure suitability for cell-based assays.
• Biological activity: Enzymatic activity may be confirmed by an NADH-coupled spectrophotometric assay; consult the CoA for lot-specific activity data.
• Formulation: The protein is provided in a defined buffer; confirm exact formulation on the product datasheet or CoA for lot rp373259.

If you require additional technical documentation, custom formulations, or bulk quantities, please contact Aladdin Scientific's technical support team.