This experiment is from the official website of Parasitology, School of Medicine, Shandong University.

Operation method

Morphological observation experiments on Leishmania donovani protozoa

Principle

Leishmania Duchenne, also known as the black fever protozoa, is mainly parasitized in human macrophages and is transmitted through the bite of the insect media, the whitefly. The developmental stage in humans is the non-flagellated body, also known as the Leishman-Donovan body, and the developmental stage in lacewings is the pro-flagellated body (flagellum). Parasitic in human and other mammals mononuclear phagocytes without flagellate body, the body is very small, oval body size of 2.9 ~ 5.7 × 1.8 ~ 4.0 um; round body diameter of 2.4 ~ 5.2 um, commonly in the macrophage. The cytoplasm of the protozoa stained with Rachel's stain was light blue or dark blue, with a large round nucleus that was red or lavender. The kinetoplast is located next to the nucleus and is darker in color, tiny, and rod-shaped (Figs. 3-7). Under the oil microscope, the body is sometimes seen to emanate a rhizoplast from the anterior granular basal body. The basal body is close to the kinetoplast and is difficult to distinguish under the light microscope. The anterior flagellum parasitizes the digestive tract of the lacewing. The mature body of the lacewing is pike-shaped, with a size of 14.3～20 um×1.5～1.8 um, the nucleus is located in the middle of the body, and the kinetoplast is in the anterior part. The basal body is in front of the kinetoplast, from which a flagellum is emitted free from the worm body (Figure 1). The anterior flagellum is motile and the flagellum is constantly oscillating. The flagellum is often aggregated into a cluster at the anterior end of the body and arranged in a daisy-like pattern within the culture medium. Sometimes it can also be seen in the form of short and thick anterior flagellum, which is related to the different degree of development.

Move

1. Live flagellomeres: These are specimens taken from Triple N's medium and observed at low or high magnification. The flagella of the anterior flagellum were often intertwined with each other, and their arrangement was often daisy-shaped (Fig. 2).



Figure 2 Leishmania dubliniensis pro-flagellar flagellum without flagellum.

2. Chinese white lacewing (Lacewing with hair): The body of the white lacewing is small, about 1/3 the size of that of a mosquito, humpbacked, and densely covered with fine, grayish-yellow hairs (for details, see section on experimental white lacewing).

3. Black fever vole autopsy specimen (bottle), compare with normal vole autopsy specimen, note the color of the internal organs and the size of the liver and spleen of the black fever vole.

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