RNase Free Grade: Overview

RNase Free grade refers to reagents, consumables, and environments that are free of ribonuclease (RNase) contamination. RNases are enzymes that degrade RNA, and they are ubiquitous—found on human skin, in dust, and on laboratory surfaces. Because RNA is fragile and highly susceptible to degradation, any trace of RNase can ruin RNA samples, leading to inaccurate experimental results.

Applications of RNase Free Reagents

RNase Free reagents are critical for any procedure involving RNA extraction, purification, analysis, or manipulation, including:

· RNA isolation (e.g., from tissue or cells)

· RT-PCR / qRT-PCR (reverse transcription polymerase chain reaction)

· Northern blotting

· RNA-Seq library preparation

· In vitro transcription

· RNA interference (RNAi) studies

Common RNase Free Reagents and Tools

Here are several types of biological reagents and materials that are available in RNase Free grade:

How RNase Free Grade is Achieved

Manufacturers ensure RNase Free grade through:

1. Chemical treatment (e.g., DEPC treatment)

2. Autoclaving to inactivate RNase enzymes

3. Ultrafiltration during purification of reagents

4. Sterile packaging in RNase Free environments

5. RNase activity testing to validate absence of contamination

Best Practices for Working RNase Free

Even when using RNase Free reagents, handling protocols are essential:

· Wear gloves and change frequently

· Use dedicated RNase Free pipettes and tips

· Clean work areas with RNase-decontamination solutions

· Avoid talking or breathing directly over open tubes

View all RNase Free products