RPAIN Antibody: Key FAQs for Research Use
RPAIN Antibody: Key FAQs for Research Use
RPAIN Antibody is a research-grade primary antibody targeting the RPA-interacting protein (RPAIN), supplied by Aladdin Scientific as product Ab351059 in a PBS-based formulation for use in standard immunological and cell biology applications.
- Product SKU
- Ab351059
- Target Protein
- RPAIN (RPA-Interacting Protein)
- Formulation
- PBS (Phosphate-Buffered Saline)
- Antibody Type
- Primary Antibody
- Supplier Reference
- absea#OC-690
- Intended Use
- Research use only (RUO)
What Is RPAIN and Why Is It an Important Research Target?
RPAIN (RPA-Interacting Protein) is a nuclear protein that associates with Replication Protein A (RPA), a heterotrimeric complex essential for DNA replication, repair, and the DNA damage response. By interacting with RPA, RPAIN is thought to play a role in modulating nuclear import of the RPA complex and in coordinating cellular responses to genotoxic stress. Research into RPAIN helps scientists better understand the regulatory networks that govern genome stability.
Studying RPAIN with a specific, validated antibody allows researchers to investigate its subcellular localization, expression levels across cell types and conditions, and its protein–protein interactions within the nucleus. These investigations are relevant to fields including cell biology, cancer biology, and DNA repair research.
Product Specifications and Formulation Details
Aladdin Scientific's RPAIN Antibody (Ab351059) is formulated in phosphate-buffered saline (PBS). PBS is a widely used, physiologically compatible buffer that helps maintain antibody stability during storage and handling. Researchers should note that this formulation does not contain carrier proteins such as BSA or preservatives such as sodium azide unless explicitly stated on the product datasheet — please consult the official product page or contact Aladdin Scientific technical support for the most current lot-specific certificate of analysis.
The supplier reference for this product is absea#OC-690. Always cross-reference this code when communicating with technical support or procurement to ensure correct product identification.
Recommended Applications and Best Practices
RPAIN Antibody is intended for research use only. Typical applications for primary antibodies of this class may include Western blotting (WB), immunofluorescence (IF), immunoprecipitation (IP), and immunohistochemistry (IHC), subject to validation data provided on the product datasheet. Researchers are strongly encouraged to review the specific application notes, recommended dilutions, and validated cell or tissue types included with each lot before beginning experiments.
For best results: (1) Centrifuge the antibody vial briefly before opening to consolidate the liquid. (2) Prepare working dilutions fresh in an appropriate diluent compatible with your assay. (3) Include appropriate positive and negative controls in every experiment to confirm antibody specificity. (4) Avoid repeated freeze–thaw cycles; aliquot the antibody upon first use if long-term storage is required.
Storage, Handling, and Stability
Proper storage is critical for maintaining antibody performance. As a general guideline for PBS-formulated antibodies, short-term storage (up to 1 month) is typically carried out at 2–8 °C, while long-term storage is recommended at −20 °C or below. Always refer to the specific storage conditions stated on the product label and certificate of analysis, as lot-specific recommendations take precedence.
To preserve antibody integrity: keep the vial tightly sealed to prevent evaporation and contamination; avoid exposure to repeated temperature fluctuations; and protect from prolonged exposure to light. If the antibody is supplied without a stabilizing carrier protein, consider adding BSA or glycerol to working aliquots for added stability during extended storage — consult Aladdin Scientific technical support for guidance.
Ordering, Returns, and Technical Support
RPAIN Antibody (Ab351059) can be ordered directly through the Aladdin Scientific website or by contacting our sales team. For bulk orders or custom requirements, please reach out to procurement support for pricing and availability. When placing an order, reference the SKU Ab351059 and supplier code absea#OC-690 to ensure accurate fulfillment.
If you have questions about product performance, experimental troubleshooting, or compatibility with specific assay systems, Aladdin Scientific's technical support team is available to assist. For returns or issues related to product quality, please consult our returns and refunds policy on the website, ensuring that requests are submitted within the applicable timeframe and accompanied by relevant documentation such as the lot number and order confirmation.
Frequently asked questions
What is the target of RPAIN Antibody (Ab351059)?
RPAIN Antibody targets RPAIN (RPA-Interacting Protein), a nuclear protein that associates with Replication Protein A (RPA) and is implicated in DNA replication and the DNA damage response. Please refer to the product datasheet for full immunogen and specificity details.
What buffer or formulation is RPAIN Antibody supplied in?
RPAIN Antibody (Ab351059) is formulated in PBS (phosphate-buffered saline). For complete formulation details including any additives or preservatives, please consult the lot-specific certificate of analysis or contact Aladdin Scientific technical support.
What applications is RPAIN Antibody validated for?
Validated applications for Ab351059 are detailed on the product datasheet. Common applications for antibodies of this type include Western blotting (WB), immunofluorescence (IF), and immunoprecipitation (IP), but researchers should confirm specific validated applications and recommended dilutions from the official product documentation before use.
How should RPAIN Antibody be stored to maintain its activity?
As a general guideline, PBS-formulated antibodies should be stored at 2–8 °C for short-term use and at −20 °C for long-term storage. Avoid repeated freeze–thaw cycles by preparing single-use aliquots. Always follow the storage instructions on the product label and certificate of analysis.
Is RPAIN Antibody suitable for clinical or diagnostic use?
No. RPAIN Antibody (Ab351059) is supplied for research use only (RUO) and is not intended for clinical diagnosis, therapeutic use, or any other non-research application. Any such use is outside the scope of the product's intended purpose.
How do I place an order for RPAIN Antibody and what is the SKU?
RPAIN Antibody can be ordered through the Aladdin Scientific website or via the sales team using SKU Ab351059. Reference the supplier code absea#OC-690 for precise identification. For bulk or custom orders, contact Aladdin Scientific procurement support directly.
References
- Mer G, Bochkarev A, Gupta R, et al. Structural basis for the recognition of DNA repair proteins UNG2, XPA, and RAD52 by replication factor RPA. Cell. 2000;103(3):449-456. doi:10.1016/S0092-8674(00)00130-3
- Wobbe CR, Weissbach L, Borowiec JA, et al. Replication of simian virus 40 origin-containing DNA in vitro with purified proteins. Proc Natl Acad Sci USA. 1987;84(7):1834-1838. doi:10.1073/pnas.84.7.1834
- Aladdin Scientific. RPAIN Antibody (Ab351059) Product Datasheet. Aladdin Scientific; [cited 2024]. Available from: https://www.aladdin-e.com
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
危险品化学品经营许可证（带存储）