This experiment is from the official website of Parasitology, School of Medicine, Shandong University.

Operation method

Experiments on morphological observation of trypanosomes

Principle

Trypanosomes can cause sleeping sickness. The trypanosomes are polymorphic and can be divided into elongated, intermediate and stubby types. The cytoplasm of trypanosomes was light blue after staining with Kirschner's or Rachel's stain, the nucleus was in the center, red or purplish red, the fluctuating membrane was light blue, and the kinetoplasts were dark red and dotted. The cytoplasm contains dark blue heterochromatin granules. The elongate type is 20-40 um long, with free flagella up to 6 μm long, and the kinetoplast is located near the end of the posterior part of the body. The short and thick type is 15～25 um, 3.5 um wide, the free flagellum is shorter than 1 um, or the flagellum is not free, and the kinetoplast is located near the posterior end of the insect. Tsetse fly is the vector. Reproduction is by dichogamy. The cone flagellum is the infective stage.

Move

Cone flagellum: cytoplasm is light blue, nucleus is medium red or purplish red, fluctuating membrane is blue, kinetoplasts are dark red, dotted, cytoplasm contains dark blue heterochromatin granules. The cytoplasm contains dark blue heterochromatin granules, but the shapes are elongated, intermediate, and short and thick. (T. rhodesiense)

Trypanosoma cruzi

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