Blood tests including blood biochemistry and ELISA require serum or plasma samples. The correct separation method can ensure the quality of serum or plasma and the correctness of the results.

Operation method

Serum and plasma preparation experiments

Materials and Instruments

Equipment:
EP tubes, thermostatic water bath, centrifuge, mice, etc.

Move

The basic procedure for serum and plasma preparation experiments can be divided into the following steps:

Whole blood obtained from the retroocular vein or abdominal a

B After standing in EP tubes at room temperature for more than 1 hour or in a 37 ℃ water bath for about 1 hour.

C Refrigerate at 4°C for 2 hours or overnight.

D Centrifuge at 3000 r/min for 10 minutes, the supernatant is serum, which can be stored at -80 ℃ after separation to avoid repeated freezing and thawing.

Caveat

1 Isolated plasma can be stored at 4 °C for several days and at -20 °C or -80 °C for longer periods. To determine lactate dehydrogenase activity, blood must be collected and measured immediately to obtain valid results.

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