Sucrose Standard Solution 10 mg/mL: Uses, Storage & FAQs
Sucrose Standard Solution 10 mg/mL: Uses, Storage & FAQs
Sucrose Standard Solution (10 mg/mL) is a precisely formulated, ready-to-use aqueous reference solution designed to support accurate calibration, method validation, and quantitative carbohydrate analysis across a wide range of analytical and biochemical applications.
- Concentration
- 10 mg/mL (10,000 µg/mL)
- SKU
- P1507806A (Archived)
- Form
- Aqueous solution, ready to use
- Primary Application
- Calibration standard for carbohydrate quantification (HPLC, colorimetric assays)
- Solute
- Sucrose (α-D-glucopyranosyl β-D-fructofuranoside)
- Storage Recommendation
- Refer to product datasheet; typically 2–8 °C, protected from light
What Is Sucrose Standard Solution (10 mg/mL)?
Sucrose Standard Solution (10 mg/mL) is a certified reference solution containing sucrose—a non-reducing disaccharide composed of glucose and fructose—dissolved in purified water at a precisely verified concentration of 10 mg per milliliter. It is manufactured and quality-controlled to provide a traceable, reproducible benchmark for analytical measurements. Because sucrose is one of the most widely analyzed carbohydrates in biological, food science, and industrial research, a reliable standard solution is essential for generating accurate, comparable data across experiments and instruments.
Aladdin Scientific offers this standard under SKU P1507806A (currently archived). Researchers should contact Aladdin's technical support team or procurement to confirm current availability or identify an equivalent in-catalog replacement.
Key Applications in the Laboratory
Sucrose Standard Solution (10 mg/mL) supports a broad range of quantitative and qualitative laboratory techniques:
- HPLC Calibration: Used as an external or internal standard for high-performance liquid chromatography methods employing refractive index (RI) or evaporative light scattering detection (ELSD) to quantify sucrose in samples such as food matrices, plant extracts, and fermentation broths.
- Colorimetric Assay Validation: Serves as the calibration standard in classical carbohydrate assays including the phenol-sulfuric acid method and the anthrone method, enabling accurate construction of standard curves.
- Enzymatic Assay Controls: Provides a defined sucrose substrate concentration for invertase activity assays and other enzyme kinetics experiments.
- Method Development and Validation: Used during assay development to assess linearity, accuracy, and precision of new analytical methods requiring a carbohydrate reference.
- Quality Control: Incorporated into laboratory QC workflows to verify instrument performance and reagent integrity on each day of use.
Storage, Stability, and Handling
Proper storage and handling are critical to maintaining the accuracy and integrity of any standard solution. The following general guidelines apply to aqueous sucrose standard solutions; always defer to the specific conditions stated on the product Certificate of Analysis (CoA) or datasheet:
- Temperature: Store at 2–8 °C in a refrigerator. Do not freeze, as freeze-thaw cycles can alter the concentration through volume change or precipitation.
- Light Protection: Keep in the original amber or foil-wrapped container if supplied, or store in darkness to prevent potential photodegradation of the solution matrix.
- Container Integrity: Always reseal tightly after use to prevent evaporation, which would increase the effective concentration and introduce error into calibrations.
- Microbial Contamination: Sucrose solutions can support microbial growth over time. Inspect the solution for turbidity or color change before each use and discard if contamination is suspected.
- Expiry: Observe the expiry date printed on the label. Do not use beyond this date for quantitative analytical work.
Note: Specific stability data for SKU P1507806A are not available in this source material. Consult the product datasheet or contact Aladdin Technical Support for confirmed shelf-life information.
Quality, Purity, and Regulatory Considerations
Standard solutions used for instrument calibration and method validation must meet defined purity and traceability requirements. While specific lot-level data for P1507806A should be obtained from the accompanying Certificate of Analysis, the following general quality considerations apply:
- Purity of Sucrose: High-quality sucrose standards are typically prepared from sucrose of ≥99% purity (often analytical or reagent grade) to minimize the contribution of impurities to measurement uncertainty.
- Concentration Accuracy: The stated concentration of 10 mg/mL should be verified gravimetrically or by a reference method during manufacture. Any uncertainty in the certified concentration should be stated on the CoA.
- Traceability: For regulated laboratory environments (e.g., GLP, GMP, ISO 17025-accredited labs), confirm whether this standard is traceable to a recognized national metrology institute (e.g., NIST, NMIJ).
- Solvent/Matrix: The standard is prepared in purified water; verify that no co-solvents, preservatives, or stabilizers are present that could interfere with your specific assay format.
Always request and review the CoA for each lot before use in critical analytical or QC workflows.
Ordering, Availability, and Support
Sucrose Standard Solution (10 mg/mL), SKU P1507806A, is currently listed as archived in Aladdin Scientific's catalog. This status indicates the product may have been discontinued, temporarily delisted, or superseded by an updated offering.
Steps for procurement:
- Contact Aladdin Scientific's customer service or technical support team to confirm whether stock remains available or whether a direct replacement SKU has been issued.
- Request the current Certificate of Analysis and Safety Data Sheet (SDS) for any lot you intend to purchase.
- If an equivalent product is required urgently, Aladdin's technical team can advise on alternative carbohydrate standard solutions within the current catalog.
For questions about payment, pricing, promotions, returns, or shipping, please refer to Aladdin Scientific's Help and Customer Service portal or contact your regional Aladdin representative directly.
Frequently asked questions
What is Sucrose Standard Solution (10 mg/mL) used for?
It is used primarily as a calibration standard in HPLC carbohydrate analysis (with RI or ELSD detection), in colorimetric assays such as the phenol-sulfuric acid method, and in enzymatic activity assays involving invertase. It can also serve as a positive control or reference material in method development and laboratory QC workflows.
How should Sucrose Standard Solution (10 mg/mL) be stored to maintain its accuracy?
The solution should generally be stored refrigerated at 2–8 °C, protected from light, and tightly sealed to prevent evaporation. Freezing is not recommended because freeze-thaw cycles can alter the effective concentration. Always check the product-specific CoA for confirmed storage conditions and expiry date.
SKU P1507806A is listed as archived — can I still purchase it?
Archived status means the product may be discontinued or temporarily unavailable. Please contact Aladdin Scientific's customer service team directly to check remaining stock or to be advised on an equivalent replacement product currently available in the catalog.
Is this sucrose standard traceable to an international metrology standard?
Traceability information is lot-specific and should be confirmed on the Certificate of Analysis supplied with each lot. For use in regulated environments (GLP, GMP, or ISO 17025-accredited laboratories), contact Aladdin Scientific's technical support to confirm the traceability status of the available lot.
Can Sucrose Standard Solution (10 mg/mL) be diluted to prepare lower-concentration working standards?
Yes, the 10 mg/mL stock solution can be diluted with the appropriate solvent (typically HPLC-grade water) to prepare a series of working standards for calibration curve construction. Dilutions should be performed gravimetrically or volumetrically with calibrated equipment, and the diluted standards should be used promptly or stored under the same conditions as the parent stock, with a shortened in-use expiry.
What analytical techniques are compatible with this sucrose standard?
This standard is compatible with HPLC using refractive index (RI) or evaporative light scattering detection (ELSD), colorimetric methods (phenol-sulfuric acid, anthrone, DNS assay), and enzymatic spectrophotometric assays. It is generally not suited for UV-absorbance-based direct detection due to sucrose's lack of a strong UV chromophore, though it may be used with post-column derivatization methods.
References
- Aladdin Scientific Product Page: Sucrose Standard Solution (10 mg/mL), SKU P1507806A. Aladdin Scientific catalog (archived listing).
- Dubois, M. et al. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry. [Phenol-sulfuric acid method reference — verify authorship and year from primary source]
- General textbook-level knowledge on sucrose chemistry, HPLC carbohydrate analysis, and standard solution preparation practices. No specific publication cited.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
危险品化学品经营许可证(带存储)