Single-Stranded DNA-Binding Proteins in Isothermal Amplification: Optimization of gp32, SSB, and Nucleic Acid Amplification Efficiency
Isothermal amplification depends on strand displacement, primer extension, template unwinding, or recombinase-mediated pairing under constant-temperature conditions. Single-stranded DNA-binding proteins can stabilize exposed ssDNA regions during amplification and reduce the effects of template ...
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