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Fluorophores are light-emitting molecules or materials that absorb energy upon excitation at specific wavelengths and emit photons at longer wavelengths. They are widely used in cell imaging, flow cytometry, immunoassays, nucleic-acid quantification, protein–protein interaction analysis, and in ...
In a broad sense, any chromophoric system that absorbs light at a shorter wavelength and re-emits it at a longer wavelength is referred to as a fluorophore. A major subgroup consists of small-molecule fluorescent dyes, but fluorophores also include fluorescent proteins and various ...
Secondary antibodies bind primary antibodies to allow detection, sorting and purification of target antigens. Since they are specific for the primary antibody species and isoform, they can detect the protein of interest. The need for a secondary antibody depends on your antibody detection method.
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