用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
Connecting multiple peptide fragments to form an extended peptide chain is referred to as ligation. Click chemistry offers a convenient method for creating peptide-peptide linkages. By introducing an alkyne group to one peptide fragment and an N-terminal azide moiety to another, a triazole ...
Click Chemistry serves as a potent technique for investigating the intracellular distribution of small molecules. Unveiling the precise cellular destinations of these compounds provides profound insights into their mechanisms of action. This methodology has been extensively employed in various ...
As a prevalent click reaction, strain-promoted alkyne-azide cycloaddition (SPAAC) operates without the need for metal catalysts, reducing agents, or enduring ligands. Instead, it harnesses the enthalpy liberated from the inherent ring strain of cyclooctyne to facilitate the formation of stable ...
SPAAC can be carried out efficiently. On the one hand, the chemical potential energy of azide and alkyne substrates as reactants is very high. Diaryl-strained-cyclooctynes, such as dibenzylcyclooctyne (DIBO), have found application in reacting with 1,3-nitrones in strain-promoted alkyne-nitrone ...
Strained alkenes leverage strain relief as a motivating factor, enabling their engagement in click reactions. Trans-cycloalkenes, typically cyclooctenes, and other strained alkenes like oxanorbornadiene, participate in click reactions with various counterparts, including azides, tetrazines, and ...
常与免疫学实验一起研究的技术。
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