用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
Western blotting is a technique that uses specific antibodies to separate proteins and is widely used to analyze the expression of specific proteins in cell or tissue extracts. This protocol details the steps for performing western blotting of cell culture and tissue samples using both ...
In cells, proteins participate in extensive protein-protein interaction networks that appear as dynamic "protein machines" that assemble and disassemble in response to a constantly changing influx of intracellular, intercellular, and extracellular cues. An initial step in understanding protein ...
RNA dot blotting is a technique similar to Northern blotting; however, RNA samples cannot be separated by electrophoresis and require membranes for imaging .
Native ChIP (N-ChIP) Protocol
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）