Flow Cytometry Using Methanol Permeabilization
Flow cytometry is a powerful technique that is often used to analyze surface and internal antigens of cells. When detecting intracellular antigens, cells usually need to be fixed and permeabilized.
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
Flow cytometry is a powerful technique that is often used to analyze surface and internal antigens of cells. When detecting intracellular antigens, cells usually need to be fixed and permeabilized.
Isotype control antibody is a negative control reagent that has the same immunoglobulin class, subclass, and light chain type as the research antibody (experimental antibody) but does not bind to the target antigen.
Procedure for early detection of apoptosis using Annexin V-FITC staining and optional propidium iodide ( PI ) .
常与免疫学实验一起研究的技术。