Protocol of Western Blotting For High Molecular Weights
High molecular weight western blotting is a modification of the standard western blotting technique specifically designed for the detection and analysis of large molecular weight proteins.
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Biological buffer
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
10 项资源 · 实验方案、常见问题与文章
Competent E. coli Cell Preparation Using the Calcium Chloride Method
Competent E. coli Cell Preparation Using the Calcium Chloride Method
Subcellular Fractionation Protocols
Subcellular fractionation is a biological experimental technique that allows researchers to separate and purify different components within cells, such as the nucleus, mitochondria, endoplasmic reticulum, etc., and then further study their structure and function. Centrifugation is one of the ...
Ethidium Bromide: Beautiful and Dangerous
Ethidium bromide (EtBr) is a classical fluorescent dye widely used in molecular biology research. Its chemical structure consists of a tri-benzimidazole, which can intercalate between the base pairs of DNA or RNA, significantly enhancing its fluorescence signal. Initially discovered as a ...
The Key to Gene Delivery to Cells —In Vitro Cell Transfection Techniques
Cell transfection techniques are the cornerstone of interdisciplinary research in molecular and cellular biology. They allow scientists to introduce foreign nucleic acids into cells to explore gene function, expression, and develop gene therapies or produce recombinant proteins. This article ...
Method and Precautions of SDS-PAGE Gel Preparation
SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a commonly used technique to separate proteins based on size. By denaturating the protein and giving it a uniform negative charge, the protein migrates to the positive pole under the application of an electric field and is ...
Guidelines For Common Cell Culture Media
Cell culture medium is an indispensable part of cell biology research, which provides essential nutrients and a suitable develope environment for cells. The following is a comprehensive introduction to the detailed components and characteristics of several commonly used cell culture media:
Native ChIP (N-ChIP) Protocol
Native ChIP (N-ChIP) Protocol
Immunocytochemistry (ICC) protocol
Immunocytochemistry (ICC) protocol
Biological buffer: the unusual amongst the usual
As a common reagent in the pharmaceutical field, biological buffer plays a crucial role in the process of regulating environmental acidity and alkalescence. With the need of epidemic prevention and control, biological buffer as a laboratory routine supplies in the fight against COVID-19 has also ...
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常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）