主题

TCA cycle

用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。

6 项资源 · 实验方案、常见问题与文章
article

Targeting Mitochondria: Intervention Strategies for Tumor Energy Metabolism

Mitochondria maintain the energy supply, biosynthesis, and redox homeostasis of tumor cells through oxidative phosphorylation, the TCA cycle, and substrate oxidation. Certain tumors and treatment-persistent cells can develop specific mitochondrial metabolic dependencies, making OXPHOS, substrate ...

更新于 2026年9月阅读12分钟
article

TCA Cycle Metabolite Detection Methods and Result Interpretation

TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...

更新于 2026年7月阅读12分钟
article

Twelve Key Enzymes in Glucose Metabolism

Glucose metabolism is a core network for cellular energy conversion and carbon allocation, spanning glycolysis, gluconeogenesis, glycogen synthesis and breakdown, the pentose phosphate pathway, and the tricarboxylic acid (TCA) cycle.

更新于 2026年1月阅读12分钟
article

Nucleotide Synthesis in Cancer Cells

In neoplastic cells, the de novo synthesis of nucleotides is responsible for maintaining sufficient nucleotide libraries to support DNA replication and RNA production. The metabolic pathways that support nucleotide production are dependent on metabolic intermediates provided by glycolysis and ...

更新于 2022年10月阅读12分钟

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常与免疫学实验一起研究的技术。