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Azide-Alkyne Click Chemistry

用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。

3 项资源 · 实验方案、常见问题与文章
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Azide-Alkyne Click Chemistry

Classic Click Chemistry uses copper, Cu(I), to catalyze the 1,3-dipolar cycloaddition of an alkyne with an azide to form a 1,2,3-triazole.1,2 Sources of Cu(I) include copper(I) iodide, copper(I) bromide, copper turnings, or copper (II) sulfate (CuSO4) and a reducing agent.1 However, the ...

更新于 2025年3月阅读12分钟
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PEG-Azide-Alkyne—Bioorthogonal and Click Chemistry

Bioorthogonal chemistry refers to a series of reactions that occur in a biological environment without affecting biomolecules or interfering with biochemical processes. For this purpose, the reaction must meet the following requirements: fast, efficient, and specific. • pH: The reaction must ...

更新于 2022年10月阅读12分钟

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