主题

Tetrazine-TCO Ligation

用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。

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protocol

Tetrazine-TCO Ligation

This ligation chemistry is based on an inverse–demand Diels–Alder cycloaddition reaction between a trans–cyclooctene and tetrazine reaction pair, forming a dihydropyridazine bond. The Tz and TCO pair shows very high reaction specificity. Its exceptional fast kinetics are reported 1~1x106 M−1s−1 ...

更新于 2025年3月阅读12分钟

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