ADC Conjugation

ADC Conjugation by DTT Reduction with Maleimide Drug Linker

Maleimide-PEG modified drug can be conjugated to the antibody at the pH 7.5 buffer solution contained 50 mM sodium phosphate, 50 mM sodium chloride and 2 mM EDTA The disulfide bridges in the antibody were then reduced with various molar equivalents of DTT at 30 ̊C for 30 min to afford different ...