Uric acid is the major end product of purine catabolism in humans and exists primarily as free uric acid or urate in blood, urine, and tissues. Uric acid quantification is a commonly used technique in clinical biochemical analysis and metabolic research and is widely applied in hyperuricemia ...
In high-performance liquid chromatography (High Performance Liquid Chromatography, HPLC), octadecylsilane-bonded stationary phase (Octadecylsilane, C18) is the most commonly used reversed-phase stationary phase. The main separation basis of C18 is hydrophobic partitioning: the more hydrophobic a ...
Hydroxyproline determination is a classical quantitative technique in collagen-related research and is widely used in fibrosis assessment, tissue-remodeling studies, collagen-material analysis, and pharmacodynamic evaluation.
Vitamin C content determination is not merely a matter of quantification, but rather a comprehensive analytical issue involving definition of the target analyte form, sample stabilization procedures, methodological selection, and the interpretive boundaries of analytical results. Because vitamin ...