Blue-white screening of bacterial colonies

Blue-White Screening of Bacterial Colonies Utilizing X-Gal and IPTG Plates

Blue-white screening of bacterial colonies is a popular and effective molecular biology tool often used to detect recombinant bacteria in cloning experiments. Central to this technique is the enzymatic activity of β-galactosidase, a tetrameric enzyme encoded by the lacZ α gene in E. coli that ...