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BRET² Assays

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Coelenterazine 400a: A High-Resolution Substrate for Advanced Bioluminescent Assays

Coelenterazine 400a is a chemically modified analog of native coelenterazine, designed to emit at shorter wavelengths (~395–400 nm) for high-sensitivity bioluminescent applications. This article explores its structural characteristics, origins, specific utility in BRET² and Ca²⁺ assays, handling ...

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