D-Luciferin In Vitro Protocol
D-Luciferin In Vitro Protocol
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D-Luciferin In Vitro Protocol
15 mg/ml Luciferin Stock Solution for In Vitro Use (D-Luciferin & L-Luciferin)
Luciferin Stock Solution for In Vivo Use
15 mg/ml D-Luciferin, Free Acid Stock Solution
D-Luciferin is a small-molecule substrate commonly used in bioluminescent reactions—most famously those observed in fireflies. When oxidized in the presence of the enzyme luciferase, along with ATP and oxygen, D-Luciferin emits visible light, typically in the yellow-green range (~560 nm).
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