The creatininase method is a commonly used quantitative method for creatinine determination in clinical biochemistry and scientific research. It mainly converts creatinine into a measurable colorimetric signal through an enzymatic cascade reaction. Compared with the alkaline picrate method, the ...
The Trinder reaction was first described by P. Trinder in the 1960s, originally as a colorimetric assay for glucose and other oxidase substrates. The method relies on the use of peroxidase (POD) to catalyze the oxidation of a chromogenic hydrogen donor in the presence of hydrogen peroxide ...