The washing step in IHC directly affects two types of outcomes. One is whether unbound or weakly bound detection reagents can be sufficiently removed. The other is whether specifically formed binding complexes can be stably retained.
Non-ionic surfactants are indispensable tools in modern science, pharmaceuticals, food technology, and cosmetics, where they function as emulsifiers, stabilizers, and solubilizers. Among them, Tween 20 (Polysorbate 20, CAS 9005-64-5) and Tween 80 (Polysorbate 80, CAS 9005-65-6) are two of the ...