Stripping, Cleaning, and Metal Recharging Workflow for Ni²⁺–Agarose Resins
In His-tag affinity purification, Ni²⁺-NTA/IDA resins gradually lose performance due to fouled coordination sites, metal loss, and pore clogging, manifested as reduced binding capacity, increased nonspecific adsorption, and elution tailing. Periodic “strip–clean–recharge” maintenance restores ...
危险品化学品经营许可证（带存储）