谷胱甘肽还原酶:维持GSH/GSSG稳态的黄素依赖还原体系及其检测与应用
谷胱甘肽还原酶(glutathione reductase,GR)是一类典型的黄素依赖型氧化还原酶,催化氧化型谷胱甘肽(GSSG)在NADPH供氢条件下还原为还原型谷胱甘肽(GSH),从而维持细胞内GSH/GSSG氧化还原缓冲体系的稳态。
用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
谷胱甘肽还原酶(glutathione reductase,GR)是一类典型的黄素依赖型氧化还原酶,催化氧化型谷胱甘肽(GSSG)在NADPH供氢条件下还原为还原型谷胱甘肽(GSH),从而维持细胞内GSH/GSSG氧化还原缓冲体系的稳态。
Glutathione reductase (GR) is a canonical flavin-dependent oxidoreductase that catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH) using NADPH as the electron donor, thereby maintaining the steady state of the intracellular GSH/GSSG redox-buffering system.
Glutathione (GSH) is one of the most important low-molecular-weight thiols in cells. It participates in reactive oxygen species (ROS) detoxification, maintains protein thiols in a reduced state, drives the glutathione peroxidase/reductase cycle, and—together with oxidized glutathione ...
谷胱甘肽(glutathione, ...
Glutathione (GSH) is a tripeptide composed of glutamate, cysteine, and glycine. It is one of the most important small-molecule reductants in cells, helping maintain redox balance, scavenge reactive oxygen species, and protect biomacromolecules from oxidative damage. Glutathione S-transferases ...
谷胱甘肽(glutathione,GSH)是一种由谷氨酸、半胱氨酸和甘氨酸构成的三肽,是细胞内最重要的小分子还原剂之一,参与维持氧化还原平衡、清除活性氧及保护生物大分子免受氧化损伤。谷胱甘肽S-转移酶(glutathione ...
常与免疫学实验一起研究的技术。