Thymidine nucleoside

EdU Staining: Basic Principle and Experimental Workflow

5-Ethynyl-2′-deoxyuridine (EdU) is a thymidine analogue incorporated into nascent DNA during S phase. Like BrdU, it labels proliferating cells, but—unlike BrdU, which requires harsh acid/base or heat denaturation to expose epitopes—EdU detection exploits its terminal alkyne for rapid, specific ...