用于检测、定量和定位抗原与抗体的技术——ELISA、免疫印迹、免疫组化与流式细胞术。以下是我们知识库中标注该主题的实验方案、常见问题与技术文章。
Stable isotope-labeled compounds use ²H, ¹³C, ¹⁵N, or ¹⁸O to label metabolic substrates, allowing their transformation and fate in vivo to be identified by mass spectrometry or nuclear magnetic resonance. This technology can be used to determine substrate sources, metabolic pathways, interorgan ...
TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...
Amino acid metabolism follows a core logic of “amino-group transfer—deamination release/assimilation—carbon-skeleton recovery—resynthesis and allocation”, spanning protein turnover, energy metabolism, and nitrogen homeostasis.
Vitamins, coenzymes, and enzymes together constitute the foundational system for cellular metabolic regulation and energy conversion.
常与免疫学实验一起研究的技术。
危险品化学品经营许可证（带存储）