FP-Biotin ABPP Protocol: Labeling & Detection of Serine Hydrolases
Step-by-step FP-biotin activity-based protein profiling protocol: probe preparation, proteome labeling, streptavidin pulldown, and SDS-PAGE/Western blot detection.
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serine hydrolase
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Lipase (Triacylglycerol Hydrolase): Interfacial Catalysis, Selectivity Classes, and Assay/Application Systems
Lipase (Lipase, EC 3.1.1.3) belongs to the carboxylic ester hydrolase family and primarily catalyzes the stepwise hydrolysis of triacylglycerols (TAGs) to diacylglycerols (DAGs), monoacylglycerols (MAGs), glycerol, and free fatty acids (FFAs).
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