The accuracy of enzyme-coupled colorimetric reactions depends on whether the roles of auxiliary enzymes are clearly defined. Mutarotase accelerates the equilibrium of sugar anomers. Oxidases and dehydrogenases convert analytes into intermediate signals such as H₂O₂ or NAD(P)H. Peroxidases then ...
The TUNEL assay is one of the most commonly used methods for detecting DNA fragmentation in apoptosis studies. Its detection target is the 3'-OH terminus of fragmented DNA rather than the apoptotic pathway itself.