This procedure is intended to standardize tissue pretreatment, fixation, sucrose cryoprotection, OCT embedding, rapid freezing, cryosectioning, section collection, frozen storage, and thawing procedures. It is applicable to immunofluorescence, immunohistochemistry, enzyme histochemistry, lipid ...
Special staining is used to visualize tissue structures, abnormal deposits, and pathogen components that are difficult to distinguish by HE staining. Its advantages include mature methodology, relatively low cost, and intuitive results, making it suitable for fibrosis evaluation, tumor ...
Lipid staining is used to visualize neutral lipid droplets, membranous lipids, cholesterol, myelin lipids, and lipid-deposition-related structures in tissue, cell, or microbial samples. Different staining methods vary greatly in target recognition, sample pretreatment, and microscopic readout.