Directions per Liter:

Dissolve 4.052g per liter of ddH2O, stirring gently until completely solubilized. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: The 100X liquid medium forms precipitates at pH values greater than 5. If higher pH is required, it is advisable to prepare a 2X solution from the stock 100X solution, and adjust this solution to the desired pH. Filter-sterilize using a 0.22 micron membrane filter. Adjust the volume accordingly for use with Thermophilus Growh Medium Base Broth.

Storage and Stability:

Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.