Background: TNFRSF21 and DR6 Biology

TNFRSF21, also known as Death Receptor 6 (DR6), is a type I transmembrane protein and member of the tumor necrosis factor receptor (TNFR) superfamily. DR6 contains multiple cysteine-rich domains in its extracellular region and an intracellular death domain, features characteristic of pro-apoptotic members of the TNFR superfamily.

DR6 has been implicated in diverse biological processes including apoptosis, T-cell activation, neurodegeneration, and axonal pruning during neural development. Research has linked TNFRSF21 signaling to activation of both caspase-8-dependent and NF-κB-mediated pathways, making it a subject of interest in oncology, immunology, and neuroscience research.

A high-quality, well-characterized monoclonal antibody such as the TNFRSF21 Mouse mAb (Ab351101) is an essential tool for detecting and studying DR6 expression, localization, and function in experimental models.

Product Specifications & Storage

Catalog Number: Ab351101
Clone Reference: absea #KC-23333
Host Species: Mouse
Antibody Class: Monoclonal Antibody (mAb)
Formulation: PBS (Phosphate-Buffered Saline) only — no carrier proteins or preservatives are listed in the supplied formulation description.

Storage Recommendations (general best practice for PBS-formulated mAbs; verify with product datasheet):

• Store at 4 °C for short-term use (up to 1 month).
• For long-term storage, aliquot and store at −20 °C or −80 °C to avoid repeated freeze-thaw cycles.
• Do not add sodium azide unless confirmed compatible with downstream assay; azide inhibits peroxidase-based detection systems.
• Avoid repeated freeze-thaw; prepare single-use aliquots upon receipt.

Note: Always refer to the official Aladdin Scientific product datasheet for lot-specific storage conditions and expiry information.

Recommended Protocol: Antibody Preparation & Handling

The following preparation and handling steps reflect general best-practice guidelines for PBS-formulated mouse monoclonal antibodies. Researchers should cross-reference the official product datasheet for lot-specific parameters.

1. Equilibration: Allow the antibody vial to equilibrate to the working temperature (4 °C or room temperature) before opening to minimize condensation-related contamination.
2. Aliquoting: Upon first use, prepare single-use aliquots in low-protein-binding microcentrifuge tubes to prevent loss of antibody through adsorption and to avoid repeated freeze-thaw cycles.
3. Dilution: Dilute the antibody in an appropriate assay buffer (e.g., PBS with 1% BSA, or TBST with 5% non-fat dry milk for Western blot blocking conditions). Optimal working dilutions must be determined empirically for each application and tissue/cell type.
4. Centrifugation (optional): Before use, briefly centrifuge the vial (1,000 × g, 30 seconds) to collect contents at the bottom and minimize loss.
5. Quality check: Inspect for visible aggregates or turbidity before use; do not use if product appears abnormal. Contact Aladdin Scientific technical support if product quality concerns arise.

Application Guidance & Troubleshooting

TNFRSF21 Mouse mAb (Ab351101) is intended for research use only. Potential applications for anti-TNFRSF21 / anti-DR6 monoclonal antibodies include, but may not be limited to, those listed below. Confirm validated applications on the official product datasheet before use.

Troubleshooting tips:

• High background: Increase blocking time or concentration; optimize wash steps.
• No signal: Verify target expression in the cell/tissue model used; confirm secondary antibody anti-mouse compatibility.
• Non-specific bands (WB): Reduce primary antibody concentration; increase stringency of washes.

All dilutions are indicative and must be optimized empirically. Verify validated applications from the official datasheet.

Safety, Regulatory & Ordering Information

Research Use Only (RUO): This product is intended solely for research purposes and is not approved for diagnostic or therapeutic use in humans or animals.

Safety: Handle all antibody preparations following standard laboratory safety protocols. While PBS-formulated antibodies without preservatives present low chemical hazard, always consult the Safety Data Sheet (SDS) available from Aladdin Scientific. Use appropriate personal protective equipment (gloves, lab coat, eye protection).

Ordering: TNFRSF21 Mouse mAb is available from Aladdin Scientific under SKU Ab351101. For volume pricing, custom bulk orders, or technical inquiries, contact your Aladdin Scientific regional representative or visit the official website.

Quality Assurance: Each lot is subject to quality control testing. Refer to the Certificate of Analysis (CoA) included with your shipment or downloadable from the Aladdin Scientific product page for lot-specific data.