Product Overview

The TRIM43B Antibody (Aladdin Scientific catalog number Ab351107, supplier reference absea#OC-437) is a primary antibody raised against the TRIM43B protein, a member of the tripartite motif (TRIM) family. TRIM family proteins are characterized by a conserved domain architecture comprising a RING finger domain, one or two B-box zinc finger domains, and a coiled-coil region. TRIM43B is implicated in cellular regulatory processes typical of the TRIM superfamily, including roles in protein ubiquitination and innate immune signaling, though its precise biological function continues to be an active area of research.

This antibody is supplied in PBS (phosphate-buffered saline) formulation without additional carrier proteins or preservatives unless otherwise specified. Researchers should verify the current formulation details on the product datasheet prior to use.

Recommended Applications & Usage Guidelines

While specific validated applications should be confirmed on the official product datasheet, antibodies in the TRIM43B class are commonly evaluated in the following experimental contexts:

• Western Blotting (WB): Use standard SDS-PAGE and transfer protocols. Optimize primary antibody dilution empirically, typically starting in the range of 1:500–1:2000. Block membranes with 5% non-fat milk or BSA in TBST prior to antibody incubation.
• Immunofluorescence (IF): Fix cells with 4% paraformaldehyde and permeabilize with 0.1–0.2% Triton X-100 as appropriate. Titrate antibody concentration to minimize background.
• Immunoprecipitation (IP): Pre-clear lysates and use appropriate protein A/G beads. Confirm target enrichment by follow-up WB.

Note: Always consult the official Ab351107 product datasheet for confirmed validated applications, species reactivity, and recommended dilution ranges, as these details supersede general guidance.

Storage, Handling & Stability

Proper storage and handling are critical to maintaining antibody performance over time. The following guidelines apply to the PBS-formulated TRIM43B Antibody (Ab351107):

• Short-term storage (up to 1 month): Store at 4°C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot upon receipt and store at −20°C or −80°C. Single-use aliquots minimize degradation from repeated thawing.
• Formulation note: As the antibody is supplied in PBS only (without stabilizers such as glycerol or BSA), adding glycerol to a final concentration of 50% (v/v) is recommended for long-term frozen storage to prevent freeze damage — confirm compatibility with the datasheet first.
• Handling: Keep on ice during experimental setup. Centrifuge briefly before opening to collect the contents. Avoid exposure to repeated temperature fluctuations.

Disclaimer: Always refer to the current product datasheet for storage conditions specific to lot Ab351107, as recommendations may be updated.

Troubleshooting Common Issues

The following troubleshooting guidance addresses common challenges encountered when working with primary antibodies in PBS formulation: