Immunological control refers to the application of various types of biological or non-biological biological agents to establish, enhance or inhibit the immune function of the organism for the purpose of preventing and treating diseases.

Operation method

Tumor protein immunopreventive assay

Materials and Instruments

Mouse
syringe

Move

The first immunization dose of general tumor protein vaccine is 10ug/mouse, mixed with the corresponding adjuvant, and injected subcutaneously on the back, the second immunization interval is 2 weeks, the same adjuvant is injected subcutaneously; the third immunization interval is 2 weeks, the same adjuvant is injected subcutaneously; 2 weeks after the third immunization, the serum potency of the vaccine is detected by ELISA, and the potency is up to the requirement; 3 days before the vaccination of the tumor cell, 20ug/mouse is injected in the peritoneal cavity or in the tail. When the serum potency reached the required level, the tumor cells were injected intraperitoneally or caudally 3 days before inoculation with tumor cells.

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