Lab Notes:

1. The fresh pH value of urine should be below 6.

2, the examined urine SG in 1.026 or less, such as <1.016 for hypotonic urine easy to destroy cell.

3, phosphate in the urine should add a small amount of glacial acetic acid, but do not add too much, so that the cell tubular lysis.

This experiment is from Mudanjiang Medical College, undergraduate 5-year clinical laboratory guide.

Operation method

Urinary Sediment Test Laboratory

Materials and Instruments

Fresh urine
Centrifuges Glass slides

Move

I. Experimental manipulation:

l. Take 10 ml of fresh urine in a test tube at 1500 r/min for 5 min.

2、After the centrifuge stops, take out the centrifuge tube and discard the supernatant. Leave 0.2ml, gently centrifuged so that the urine sediment is mixed with the formation of components.

3、Take the precipitate on a glass slide for microscopic examination．

Second, the results of the experiment to determine: 10 × 10 times, observe which have formed a fraction of the tube type, 10 × 40 times to observe the cellular composition and counting, observation of 10 fields of vision tube type counting 20 fields of vision.

Reference value:

1, cellular components: minimum - maximum value / HP

RBL 0-3/HP WBL 0-5 cells/HP

2. Tube type (transparent): average value/HP

3、The number of urine crystals and salts is reported in each high power field of view (+) (2+) (3+)

4, specimen collection: the patient first urinate to discard the accurate collection of 3h urine in a clean desiccator (specimen retention 5:30-8:30).

5, accurate measurement of 3 hours of urine volume, fully mixed urine l0ml, 1500r/min 5 minutes, with a pipette to absorb the upper layer of urine 9ml retained 1ml, absorb 1 drop of mixed urine injected into the counting disk, Cal counting 10 large squares, 20 large squares of the tube.

6, l hour cell = 10 large square cell total × (100O/10) × (3h urine volume / 3)

1 hour Cost count = (20 large square cells tube type number/2) x (10O0/10) x (3h try/3)

Where 1000 is the number of microliters converted to milliliters and 10 is the number of times the urine is concentrated.

Caveat

l, early morning fasting for the first time the urine should be sent within lh test2、Preparation of clean, dry urine cup, retained in the mid-range urine, urine cell and tube count. addis two-hour urine sediment count.3, fresh urine PH value should be below 6.4, the examined urine SG in 1.026 or less, such as ＜1.016 for hypotonic urine easy to destroy cell.5, phosphate in the urine should be added a small amount of glacial acetic acid, but do not add too much, so that the cell tubular dissolution.

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