Application of microcomputer control, the use of reflective colorimetric principle to determine the results of dry chemical test strip reaction analyzer, this experiment from the Mudanjiang Medical College undergraduate 5-year clinical laboratory guide

Operation method

Urine automatic analyzer operation experiment

Principle

Application of microcomputer control, the use of reflective colorimetric principle to determine the results of dry chemical test strip reaction analyzer. Instrument structure: optical system (spherical integrator), scanning structure. Current / voltage converter (l / V), central processing unit (CPu) control mechanism. Auxiliary printers, monitors, operating kits.

Move

After the urine-soaked reagent tape is put into the detection tank of the instrument, the optical drive section scans the reagent tape, the reagent is irradiated by tungsten lamp, the reflected light is pooled and homogenized in the spherical integrator, and filtered by a fixed wavelength filter, so that the photoelectric tube receives dual wavelength irradiation of the measured light and a beam of reference light, and converts the optical signal into an electrical signal, and at the same time, a blank film block with urine soaked without reagent is used as the background color of the urine for color correction. At the same time by the wet urine without reagent blank membrane block for urine background color compensation to correct the error generated by the instrument change factor and urine color, the signal is then converted by the current/voltage converter and input into the central processor, the reflectance of the measurement is calculated according to the following formula, and then compared with the standard value of the reflectance of the storage, automatically calculate and print out the semi-quantitative grade symbols of the test components and the concentration of the expressed value:

Reflectance formula:

R (%) = (Tm.Cs/Ts.Cm)×l00%

R (%) is the reflectivity: Tm is the reflective intensity of the reagent film block at the measured wavelength.

Ts is the reflection intensity of the reagent film block to the reference wavelength.

Cm is the reflection intensity of the calibration film block to the measurement wavelength.

Cs is the reflected intensity of the calibration module to the reference wavelength

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