Examination of urinary ferritin in patients with intravascular hemolysis. This experiment was obtained from the laboratory instruction of Mudanjiang Medical College undergraduate 5-year testing program.

Operation method

Urine ferritin stain Rous test

Principle

The same principle as the Prussian blue reaction for iron staining of bone marrow.

Materials and Instruments

urine
bone marrow stain

Move

Urine centrifugation precipitation, pour off most of the supernatant, the sediment at the bottom of the tube shaking well, add lml 4% acidic low ferricyanide potassium role at room temperature for 10 minutes when shaking, and then centrifugation precipitation, take the precipitate l drop placed on the slide, plus cover slips and then high magnification microscopic examination, the other method will be the urine sediment for the smear first, dry according to the bone marrow iron staining method of staining examination of the high magnification examination of the urine ferricytohematocrit is a free blue rounded small particles or in the Epithelial cells, such as found in the epithelial cells. The significance of this is even greater, because the artificial blue precipitate in the iron staining process does not exist in the cells.

Caveat

This test is mostly positive in patients with episodes of intravascular hemolytic reaction. It is particularly informative in the diagnosis of paroxysmal sleep hemoglobinuria.

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