What are the considerations for testing tissue samples for intracellular indicators?

① All sample tubes need to be fixed and membrane-broken in the same way as the experimental groups, such as blank control, single-label group, fmo-iso; 

② Fixed and membrane-broken time of each group should not be too long difference, and it is recommended to operate in batches if the sample volume is large; 

③ For the tumor tissue samples, it is necessary to consider increasing the dosage of antibody due to the small amount of the target cells to be detected and the number of uptime should be at least 1 million; 

④ It is recommended to increase the number of 75 μm micron It is recommended to add 75um micron pore size filter for secondary filtration to improve the purity and content of immune cells; ⑤ Due to the long stimulation blocking time, it is necessary to pay attention to the aseptic operation before the end of the stimulation blocking time.

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