What are the precautions for human peripheral blood testing for intracellular factor?

Choose heparin anticoagulation tube; ② Sample preparation: a. Human peripheral blood samples: dilute erythrocyte lysate in advance and use it now; b. PBMC samples: rewarm the samples and the reagents to 18~20℃ before performing the experiments; the experimental ambient temperature is controlled at 18~20℃; try to separate the PBMC from the collected fresh human blood within 1 hour; avoid washing out the layered liquid surface when adding blood; do not mix or shake the tubes after adding blood; avoid mixing or shaking the tube. mix or shake the tube. During blood collection, blood samples should be shaken well in heparin anticoagulation tubes to avoid coagulation (the storage time of heparin anticoagulation tubes should not be more than 12 h in general); ④ The time of red cracking should not be more than 5 min, and it is recommended to do the pre-experiment to feel out the optimal time; ⑤ Aseptic operation; ⑥ Stimulation and blocking time are recommended to feel out by the pre-experiment.

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