What related reagents are needed for flow-through staining?

Samples with red blood cells, need to use red blood cell lysate, lysed red blood cells;
Fc receptor-rich cells such as macrophages need to be closed to FC receptor before adding the flow antibody to minimize non-specific binding of the antibody.
Cell staining buffer is required for the cell staining process;
Fixed rupture solution for intracellular indicator detection is required for intracellular indicator detection, and fixed rupture solution for intranuclear indicator detection is required for intranuclear indicator detection;
Dead cell dyes are also used for flow-through assays on tissue samples such as tumors.

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