Thanos Poly Active PCR Mix 是一款含热启动抗体酶的荧光定量预混液，不仅可支持含引物探针全预混，且对于DNA多重项目适配性强。为减少终端的繁琐实验操作，可提前配好含引探预混液，检测时直接加入样本即可。
本产品使用热启动抗体酶搭配针对全预混体系独家研发的缓 冲Buffer，极大提升了扩增体系的稳定性和DNA样本的检测灵敏度。并于呼吸道、腹泻、HPV、兽用病原体检测等类型qPCR项目 上适配性强，通用性佳。
产品号 | 组分 | 100T | 1000T | 10000T | 存储温度 | FP1508890 | UltraBio™ Thanos Poly Active PCR Mix | 1.6 mL | 10×1.6 mL | 160mL | -20℃ |
|
适用范围： 以 DNA 为模板的核酸定量或定性检测，只需要添加引物探针，补足 ddH₂O 即可。
产品优势：
1. 全新研发的Buffer和热启动抗体酶组合，确保反应的高特异 性和高灵敏度；
2. 加入引探即可制成成品预混液，检测时加入样本即可；
3. 引入dUTP/UDG防污染系统，可有效防止气溶胶污染；
4. 可适配快速和多重靶标扩增。
使用说明
反应体系配制：
1. 溶解并混匀 PCR 反应所需的各种溶液，瞬时离心后，放置室温待用，探针建议避光处理。建议 PCR 液体分装使用，避免反复冻融。
2. 参照下表配制 PCR 反应体系：
组分
| 加入体积/25μL
| 加入体积/50μL 体系
| 终浓度
|
Thanos Poly Active PCR Mix
| 16 μL
| 32 μL
| 1× |
引物探针 Mix
| 1 μL
| 2 μL
| / |
模板
| 5 μL
| 10 μL
| /
|
ddH₂O
| To 25 μL
| To 50 μL
| /
|
终体积
| 25 μL
| 50 μL
|
|
注 1：引物以及模板的用量可自行调整
注 2：使用快速反应程序时，建议将反应体系总体积设置为 25μL
3. 用移液器轻轻吹打混匀或轻微 Vortex 混匀，室温离心数秒，使液体积聚于管底。
4. 将各 PCR 反应管置于设置好的 PCR 仪上，开始 PCR 反应。
步骤名称
| 温度
| 时间
| 循环 |
预变性
| 95℃
| 1min 30s
| 1 |
变性
| 95℃
| 5s
| 40 |
退火
| 55℃
| 30s（收集荧光）
| 40 |
注：反应条件可根据实际情况自行调整。
Thanos Poly Active PCR Mix is a fluorescent quantitative premix containing hot-start antibody enzymes. It not only supports full premixing with primers and probes but also exhibits strong adaptability for multiplex DNA assays. To reduce tedious experimental operations at the end-user stage, the primer-probe premix can be prepared in advance, and samples can be directly added during detection.This product uses hot-start antibody enzymes combined with an exclusively developed buffer for the full premix system. It greatly enhances the stability of the amplification system and the detection sensitivity of DNA samples. It also shows strong adaptability and versatility in qPCR assays such as respiratory tract, diarrhea, HPV, and veterinary pathogen detection.
Catalog | Component | 100T | 1000T | 10000T | Storage | FP1508890 | UltraBio™ Thanos Poly Active PCR Mix | 1.6 mL | 10×1.6 mL | 160mL | -20℃ |
|
Application Scope
Quantitative or qualitative nucleic acid detection using DNA as the template. Only primers, probes, and supplementary ddH2O need to be added.
Product Advantages
- Novel combination of independently developed buffer and hot-start antibody enzymes ensures high specificity and sensitivity of reactions.
- Ready-to-use working premix can be prepared by simply adding primers and probes; samples are added directly during detection.
- Integrated dUTP/UDG anti-contamination system effectively prevents aerosol contamination.
- Compatible with rapid amplification and multiplex target amplification.
Usage Instructions
Reaction System Preparation
1. Dissolve and mix all solutions required for the PCR reaction thoroughly. After brief centrifugation, place at room temperature for later use. Probes should be protected from light. It is recommended to aliquot PCR solutions for use to avoid repeated freeze-thaw cycles.
2. Prepare the PCR reaction system according to the following table:
Component
| Volume per 25 µL Reaction | Volume per 50 µL Reaction | Final Concentration
|
Thanos Poly Active PCR Mix
| 16 μL
| 32 μL
| 1× |
Primer/Probe Mix
| 1 μL
| 2 μL
| / |
Template DNA
| 5 μL
| 10 μL
| /
|
ddH₂O
| To 25 μL
| To 50 μL
| /
|
Total Volume
| 25 μL
| 50 μL
|
|
Note 1: The amounts of primers and template can be adjusted as needed.
Note 2: When using a rapid reaction program, it is recommended to set the total volume of the reaction system to 25μL.
3. Gently mix by pipetting up and down or slight vortexing. Centrifuge at room temperature for a few seconds to collect the liquid at the bottom of the tube.
4. Place each PCR reaction tube on the pre-set PCR instrument and start the PCR reaction.
| Step | Temperature
| Time | Cycles
|
Pre-denaturation
| 95℃
| 1min 30s
| 1 |
Denaturation
| 95℃
| 5s
| 40 |
Annealing/Extension
| 55℃
| 30s (Acquire fluorescence)
| 40 |
Note: The reaction conditions can be adjusted according to actual circumstances.
ZJ26F0333675
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