计算溶液所需的质量、体积或浓度。
BioReagent,for DNA and RNA applications,PCR试剂,分子生物学级,无DNA酶和RNA酶 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
UltraBio™ 2×Taq Master Mix (Dye Plus) 专为定性PCR设计,带有电泳保护剂和溴酚蓝染料,可在反应结束后直接电泳。本产品含热启动的Taq DNA Polymerase、dNTP mix和含镁离子的缓冲体系,只需加入模板和引物探针即可扩增,扩增产物可直接进行琼脂糖凝胶电泳分析。本产品在保证扩增效率和特异性的同时简化了操作流程,节省反应时间。
适用范围:带有产物分析步骤的定性 PCR。
使用说明
1. 反应体系配制
a. 室温或 4℃溶解反应所需试剂,混匀后备用;
b. 参考下表设置 50 μL 反应体系:
| 组分 | 加入体积 | 终浓度 |
| 2×Taq Master Mix (Dye Plus) | 25 μL | 1× |
| 引物 ※ | — | — |
| 样本 ※ | — | — |
| ddH₂O | To 50 μL | — |
| 终体积 | 50 μL | — |
※ 引物和样本按项目需求添加;
c. 建议反应液体分装使用,避免反复冻融;
d. 建议反应体系的配制在冰浴或在冰盒上进行。
2. 反应程序

注意事项
因本产品带有用于琼脂糖凝胶电泳的溴酚蓝染料,溶液呈蓝紫色,使用前请充分振荡混匀。
UltraBio™ 2×Taq Master Mix (Dye Plus) is specifically designed for qualitative PCR. It contains a loading dye (bromophenol blue) and a gel electrophoresis buffer, allowing direct loading of the PCR reaction onto an agarose gel without additional steps. This ready-to-use mix includes hot-start Taq DNA Polymerase, dNTPs, and optimized buffer with magnesium. Users only need to add template and primers to perform amplification, and the products can be directly analyzed by agarose gel electrophoresis. This product ensures high amplification efficiency and specificity while simplifying operation and saving time.
Intended Use: Qualitative PCR requiring post-amplification analysis.
Instructions for Use
1. Preparation of Reaction Mix
a. Thaw all reagents at room temperature or on ice and mix gently before use.
b. Prepare a 50 µL reaction system as follows:
| Component | Volume | Final Concentration |
| 2×Taq Master Mix (Dye Plus) | 25 μL | 1× |
| ※ Primers | — | — |
| ※ Sample | — | — |
| ddH₂O | To 50 μL | — |
| Total Volume | 50 μL | — |
※ Add primers and sample according to project requirements.
c. It is recommended to aliquot the reaction mix to avoid repeated freeze-thaw cycles.
d. Prepare the reaction mix on ice or a cooling rack.
2. Thermal Cycling Protocol

Note
Because this product contains bromophenol blue dye for agarose gel electrophoresis, the solution appears bluish‑purple. Mix thoroughly by vortexing before use.
通过匹配包装上的批号来查找并下载产品的 COA,每批产品都进行了严格的验证,您可放心使用!
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | FP1509090 |
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 DNase, RNase free 级别指南 → 查看 PCR Reagent 级别指南 → 查看 Suitable for molecular biology 级别指南 → 查看 for DNA and RNA applications 级别指南 →