计算溶液所需的质量、体积或浓度。
BioReagent,PCR试剂,分子生物学级,无DNA酶和RNA酶 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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UltraBio™ 2× Taq SYBR Green Premix (Universal) 是使用SYBR Green I嵌合荧光法进行qPCR反应的DNA预混液。使用抗体修饰的热启动抗体酶AK Taq V2 Polymerase,结合特殊调试的Buffer,具有特异性强,扩增活性佳,检测灵敏度高等优势,
本产品不含ROX reference Dye。
适用范围:以 DNA 为模板的核酸定量或定性检测,只需要添加引物,补足ddH₂O即可。
产品优势
1、全新研发的Buffer和热启动抗体酶组合,确保体系的高扩增效率和高灵敏度;
2、针对SYBR荧光染料方法学进行特殊调试,大大提高了体系对样本的特异性扩增;
3、加入引物和模板即可扩增,简单快捷。
使用说明
1、反应体系配制:
1.1 溶解并混匀 PCR 反应所需的各种溶液,瞬时离心后,放置室温待用。建议 PCR 液体分装使用,避免反复冻融。
1.2 参照下表配制 PCR 反应体系:
| 组分 | 加入体积/20μL 体系 | 加入体积/40μL 体系 | 终浓度 |
| UltraBio™ 2× Taq SYBR Green Premix (Universal) | 10 μL | 20 μL | 1× |
| ROX Reference Dye(50×) | 0.4 | 0.8 | 1× |
| 引物 Mix | 1 μL | 2 μL | / |
| 模板 | 4 μL | 8 μL | / |
| ddH₂O | To 20 μL | To 40 μL | / |
| 终体积 | 20 μL | 40 μL |
注1:引物以及模板的用量可自行调整;
注2:ROX Reference Dye(50×)需自行采购,根据不同机型使用高浓度/低浓度ROX染料。
1.3 由于体系较为粘稠,用移液器轻轻吹打混匀或 Vortex 充分混匀,室温离心数秒,使液体积聚于管底。
1.4 将各 PCR 反应管置于设置好的 PCR 仪上,开始 PCR 反应。
反应程序

注:反应条件可根据实际情况自行调整
注意事项
收到试剂后可分装使用,避免反复冻融。
UltraBio™ 2× Taq SYBR Green Premix (Universal) is a DNA premix for qPCR reactions utilizing the SYBR Green I chimeric fluorescence method. It employs the antibody-modified hot-start enzyme AK Taq V2 Polymerase, combined with a specially optimized buffer, offering advantages such as high specificity, excellent amplification efficiency, and high detection sensitivity.
This product does not contain ROX reference dye.
Product Advantages
1. A newly developed buffer and hot-start antibody-modified enzyme combination ensures high amplification efficiency and high sensitivity of the system.
2. Specially optimized for the SYBR green dye methodology, significantly improving the system's specificity for sample amplification.
3. Simple and fast amplification – just add primers and template.
Instructions for Use
1. Reaction Setup:
1.1 Thaw, mix thoroughly, and briefly centrifuge all solutions required for the PCR reaction. Keep them at room temperature for use. It is recommended to aliquot PCR reagents to avoid repeated freeze-thaw cycles.
1.2 Prepare the PCR reaction system according to the table below:
| Component | Volume / 20 μL Reaction | Volume / 40 μL Reaction | Final Concentration |
| UltraBio™ 2× Taq SYBR Green Premix (Universal) | 10 μL | 20 μL | 1× |
| ROX Reference Dye(50×) | 0.4 | 0.8 | 1× |
| Primer Mix (Forward & Reverse) | 1 μL | 2 μL | / |
| Template DNA | 4 μL | 8 μL | / |
| ddH₂O | To 20 μL | To 40 μL | / |
| Total Volume | 20 μL | 40 μL |
Note 1: The amount of primers and template can be adjusted as needed.
Note 2: ROX Reference Dye (50×) must be purchased separately. Use high or low concentration ROX dye according to the instrument requirements.
1.3 Due to the viscosity of the mix, gently pipette to mix or vortex thoroughly. Briefly centrifuge at room temperature to collect the liquid at the bottom of the tube.
1.4 Place the PCR tubes on a pre-programmed thermal cycler and start the PCR reaction.
Reaction Program:

Note: The reaction conditions can be adjusted based on primer Tm, amplicon length, and instrument requirements.
Precautions
After receiving the reagent, it can be aliquoted for use to avoid repeated freeze-thaw cycles.
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