UltraBio™ Taq Platinum Universal SYBR Green Premix (2×, ROX Separated) 是使用SYBR Green I嵌合荧光法进行qPCR反应的DNA预混液。使用抗体修饰的热启动抗体酶AK Taq V2 Polymerase，结合特殊调试的Buffer，具有特异性强，扩增活性佳，检测灵敏度高等优势。
适用范围：以 DNA 为模板的核酸定量或定性检测，只需要添加引物，补足ddH2O 即可。
产品组分表
FP1509096 | 组分 | 100T | 1000T | 10000T | 储存温度 |
FP1509096A | 2× Taq Platinum Universal SYBR Green Premix | 1 mL | 10×1 mL | 100mL | -20℃.Store in the dark. |
FP1509096B | 50× ROX Reference Dye1 | 40 μL | 400 μL | 4mL | -20℃.Store in the dark. |
FP1509096C | 50× ROX Reference Dye2 | 40 μL | 400 μL | 4mL | -20℃.Store in the dark. |
产品优势：
1、全新研发的Buffer和热启动抗体酶组合，确保体系的高扩增效率和高灵敏度；
2、针对SYBR荧光染料方法学进行特殊调试，大大提高了体系对样本的特异性扩增；
3、加入引物和模板即可扩增，简单快捷。
使用说明
1、反应体系配制：
1.1 溶解并混匀 PCR 反应所需的各种溶液，瞬时离心后，放置室温待用。建议 PCR 液体分装使用，避免反复冻融。
1.2 参照下表配制 PCR 反应体系：
组分
| 加入体积/20μL 体系
| 加入体积/40μL 体系
| 终浓度
|
2× Taq Platinum
Universal SYBR Green Premix
| 10 μL
| 20 μL
| 1×
|
| 50× ROX Reference Dye1 (or Dye2) | 0.4
| 0.8
| 1×
|
引物 Mix
| 1 μL
| 2 μL
| /
|
模板
| 4 μL
| 8 μL
| /
|
ddH2O
| To 20 μL
| To 40 μL
| /
|
终体积
| 20 μL
| 40 μL
|
|
注1：引物以及模板的用量可自行调整
注2：
ABI PRISM 7000/7300/7900HT and 7900HT Fast，ABI Step One，ABI Step One Plus等需要高浓度ROX染料的机型使用50× ROX ReferenceDye1；
ABI 7500，7500 Fast，等需要低浓度 ROX 染料的机型使用 50× ROX Reference Dye2。
1.3 由于体系较为粘稠，用移液器轻轻吹打混匀或 Vortex 充分混匀，室温离心数秒，使液体积聚于管底。
1.4 将各 PCR 反应管置于设置好的 PCR 仪上，开始 PCR 反应。
反应程序
注：反应条件可根据实际情况自行调整
注意事项
收到试剂后可分装使用，避免反复冻融。
UltraBio™ 2× Taq Platinum Universal SYBR Green Premix (ROX Separated) is a ready-to-use master mix optimized for quantitative PCR (qPCR) using SYBR Green I dye-based chemistry. It incorporates antibody-mediated hot-start AK Taq V2 Polymerase and a specially formulated buffer, ensuring high specificity, excellent amplification efficiency, and superior detection sensitivity.
This product includes separate vials of ROX reference dye, making it compatible with a wide range of real-time PCR instruments. Users only need to add primers and template to perform the reaction.
Intended Use: Quantitative or qualitative detection of nucleic acids using DNA templates. Simply add primers and RNase-free water to complete the reaction.
Product Component Table
FP1509096 | Components | 100T | 1000T | 10000T | Storage |
FP1509096A | 2× Taq Platinum Universal SYBR Green Premix | 1 mL | 10×1 mL | 100mL | -20℃. Store in the dark. |
FP1509096B | 50× ROX Reference Dye1 | 40 μL | 400 μL | 4mL | -20℃.Store in the dark. |
FP1509096C | 50× ROX Reference Dye2 | 40 μL | 400 μL | 4mL | -20℃. Store in the dark. |
Key Features
1. High Efficiency & Sensitivity: A novel buffer system combined with a hot-start antibody-modified polymerase ensures high amplification efficiency and sensitivity.
2. Enhanced Specificity: Specially optimized for SYBR Green I chemistry, significantly improving amplification specificity.
3. Simple & Fast: Just add primers and template—no other components are needed.
Protocol
1. Reaction Setup
1.1 Thaw and mix all PCR reagents thoroughly. Briefly centrifuge to bring the solution to the bottom of the tube. It is recommended to aliquot the master mix to avoid repeated freeze-thaw cycles.
1.2 Prepare the PCR reaction according to the table below:
Component
| Volume per 20 µL Reaction
| Volume per 40 µL Reaction
| Final Concentration
|
2× Taq Platinum
Universal SYBR Green Premix
| 10 μL
| 20 μL
| 1×
|
| 50× ROX Reference Dye1 (or Dye2) | 0.4
| 0.8
| 1×
|
Primer Mix
| 1 μL
| 2 μL
| /
|
Template DNA
| 4 μL
| 8 μL
| /
|
ddH2O
| To 20 μL
| To 40 μL
| /
|
Total Volume
| 20 μL
| 40 μL
|
|
Note 1: The amount of primers and template can be adjusted according to experimental needs.
Note 2: 50× ROX Reference Dye 1 is recommended for instruments requiring a high concentration of ROX, such as ABI PRISM® 7000/7300/7900HT, 7900HT Fast, StepOne, and StepOnePlus. 50× ROX Reference Dye 2 is recommended for instruments requiring a low concentration of ROX, such as ABI 7500 and 7500 Fast.
1.3 Mix thoroughly by pipetting gently or vortexing, as the solution is viscous. Briefly centrifuge to collect the liquid at the bottom of the tube.
1.4 Place the reaction tubes in a pre-programmed real-time PCR instrument and start the run.
2. Thermal Cycling Protocol
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Note: The reaction conditions can be adjusted based on primer Tm, amplicon length, and instrument requirements.
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